RNA

Part:BBa_K3467001:Design

Designed by: Wang Ran   Group: iGEM20_CAU_China   (2020-10-21)


TSP dsRNA sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Considering with the bio-safety, the dsRNA sequence should avoid other species and has a high identity to the target locust TSP mRNA to prevent it from off-target.


Source

TSP dsRNA sequence is from Locusta migratoria cDNA. We extract the dsRNA sequence by using PCR with specific primers.

References