RNA
Part:BBa_K3467001:Design
Designed by: Wang Ran Group: iGEM20_CAU_China (2020-10-21)
TSP dsRNA sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Considering with the bio-safety, the dsRNA sequence should avoid other species and has a high identity to the target locust TSP mRNA to prevent it from off-target.
Source
TSP dsRNA sequence is from Locusta migratoria cDNA. We extract the dsRNA sequence by using PCR with specific primers.